Dissociation of aggregated IgG and denaturation of monomeric IgG by acid treatment.
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چکیده
منابع مشابه
Association and dissociation of aggregated IgG from rat peritoneal macrophages
Stable aggregated IgG (A-IgG) of various sizes, having high biological activity, were incubated at 4 degree C with adhering peritoneal macrophages from normal rats and the kinetics of A-IgG binding to the cell surface were studied. Equilibrium constants were high (2.8-11.7 X 10(8) M-1) and varied as a function of aggregate size. The maximum number of A-IgG bound per cell varied from 230,000 for...
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An autoradiographic binding assay employing (125)I-labeled heat-aggregated mouse IgG2b myeloma protein (MOPC 141) was used to demonstrate receptors for IgG on 20-45% of Balb/c thymocytes and on 70-80% of splenocytes. Binding could also be shown with heat or BDB aggregates of another IgG2b (MOPC 195), with IgG1 and with human gamma-globulin, but not with aggregated chicken gamma-globulin, IgA, B...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1983
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.141.337